The Premier League announced that club's themselves could decide whether or not to bar the instruments, which caused controversy during this summer's World Cup in South Africa.
"Following discussions with the police and representatives from the local licensing authorities, the club will not be permitting vuvuzelas or similar instruments into White Hart Lane on match days," a statement on the club's official website read.
"We are concerned that the presence of the instruments within the stadium pose unnecessary risks to public safety and could impact on the ability of all supporters to hear any emergency safety announcements.
"We are very proud of the fantastic atmosphere that our supporters produce organically at White Hart Lane and we are all very much looking forward to this continuing into the forthcoming season."