A first-half goal from Atletico's Diego Godin proved enough to ensure their second defeat from as many games in the competition
They lost their opening fixture 2-1 to Juventus at the same venue.
Spurs manager Mauricio Pochettino began with both of his new signings Vincent Janssen and Victor Wanyama in a weakened XI
Erik Lamela and Christian Eriksen were the only two regular starters who began, but encouragingly the £17million Janssen showed promise in the absence of Harry Kane
Atletico were also not at full strength, but Jan Oblak, Godin, Stefan Savic, Filipe Luis, Gabi, Saul Niguez and Fernando Torres were involved
Eriksen tested Atletico goalkeeper Oblak with a dangerous seventh-minute shot
Then in the 21st minute Lamela, the goalscorer against Juve, played a one-two with Janssen before striking the inside of the post
Atletico's goal came in the 40th minute, and after the lively Torres had already threatened
Spurs' Harry Winks fouled Thomas Partey and from the resulting free-kick Gabi's delivery came off Anton Walkes to leave Godin with a two-yard tap-in
Janssen caught the eye in the second half, when combining with substitute Son Heung-min to play in Ryan Mason, whose powerful shot was saved by Oblak
From a 62nd-minute free-kick, the Dutchman then impressively curled towards the bottom corner but the authoritative Oblak produced another fine save
Tom Carroll and Nacer Chadli made substitute appearances, but despite improving on their performance against Juve, Spurs were unable to find an equaliser
Source : PA
Source: PA