Manager Harry Redknapp has confirmed that he Spurs and England international striker has suffered a grade-two tear and will be sidelined for 'a few weeks'.
"Defoe has just torn a hamstring, in training yesterday,' revealed Redknapp.
"I'm not sure how long it will be. It's a grade two tear, so it will be a few weeks so he's struggling. He'll be back but it will be a few weeks before he's fit.
"We're okay in attack, I've got Peter Crouch and Eidur Gudjohnsen who can come in. It's other areas in which we're short," Reknapp added.
"David Bentley went down with a bit of a virus this morning and Tom (Huddlestone) is not fit.
"Aaron Lennon is raring to go and wants to get out and give it a go again, but he's not ready yet. He's desperate to play and we're desperate to get him back but he's still way off yet.
"We've run in to a bad run of injuries."